Table 1 Production of phenylpropanoic acids by recombinant E. coli strains.

From: Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources

E. coli BL21(DE3)

Product (mg/L)

4-Coumaric acid

Caffeic acid

Ferulic acid

tal/pACYC

821 ± 31a

—

—

sam8/pACYC

875 ± 46a

—

—

tal-hpaBC/pACYC

272 ± 17

44 ± 13b

—

sam8-sam5/pACYC

221 ± 34

136 ± 6b

—

tal-hpaBC-comt/pACYC

112 ± 21

41 ± 10

43 ± 9c

sam8-sam5-comt/pACYC

86 ± 11

37 ± 13

156 ± 12c

  1. The recombinant E. coli strains were cultured in LB medium with 1.5 g/L l-tyrosine. The sign ‘—’ indicated that the compound not detected in HPLC analysis. The data are generated from at least triplicate independent experiments, and the standard deviations were shown. Different letter codes (a, b and c) indicate significant differences (a, P = 0.061; b, P = 0.00037; c, P = 0.0002).
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