Figure 3

miR-33a negatively regulates Twist1 expression.
Quantitative RT-PCRs of Twist1 mRNA expressions in SPC-A-1 and NCI-H1299 cells were performed after transfections of anti-miR-33a (A) and miR-33a (B). Western blotting (C,D) and semi-quantitative analyses (E,F) of Twist1 protein in SPC-A-1 and NCI-H1299 cells treated as described above. *P < 0.05, **P < 0.01.