Figure 4 | Scientific Reports

Figure 4

From: Compensatory T-type Ca2+ channel activity alters D2-autoreceptor responses of Substantia nigra dopamine neurons from Cav1.3 L-type Ca2+ channel KO mice

Figure 4

Age-dependent T type Ca2+ channel modulation of pacemaker activity in SN DA neurons.

(a/b) Exemplary perforated patch-clamp or cell-attached recordings of spontaneous activity of SN DA neurons from juvenile (a) and adult (b) WT mice without and with T-type channel inhibitor Z941 (pre-)incubation (10 μM, indicated by purple bars). Under each original 10 second trace, a 30 seconds schematic spike train representation is given. Arrows point to irregularity in pacemaker-activity in SN DA neurons from juvenile WT mice in Z941. (c) Data analysis as in Fig. 2 (d) Left: Mean SN DA pacemaker frequencies. Note that Z941 significantly reduced SN DA pacemaker frequency of adult WT SN DA but not of juvenile WT SN DA neuron. Right: Mean SN DA pacemaker precision, given as CV2 values. Note that Z941 significantly reduced pacemaker precision of juvenile WT SN DA but not adult WT SN DA neurons. All data are shown as mean ± SEM. Significant differences are marked by asterisks. Data values are detailed in Table 1.

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