Table 1 AHP, pacemaker frequency and its precision, as well as D2-AR responses in SN DA neurons from WT and Cav1.3 KO mice.

From: Compensatory T-type Ca2+ channel activity alters D2-autoreceptor responses of Substantia nigra dopamine neurons from Cav1.3 L-type Ca2+ channel KO mice

 

control conditions

control conditions

juvenile WT

n

juvenile CaV1.3 KO

n

U

p

adult WT

n

adult CaV1.3 KO

n

U

p

afterhyperpolarisation [mV] (whole-cell)

−59.27 ± 0.6

47

−55.25 ± 0.9

40

560

0.001

−56.52 ± 0.8

46

−59.75 ± 1.1

22

327

0.02

pacemaker frequency [Hz] (five minutes, perforated patch)

2.67 ± 0.3

7

2.07 ± 0.3

9

15.5

0.1

3.18 ± 0.2

9

3.34 ± 0.3

13

55

0.8

CV ISI [%] (perforated patch)

7.71 ± 0.9

7

16.77 ± 4

9

15

0.09

7.13 ± 2

5

11.36 ± 2.3

6

6

0.1

CV2 [%] (perforated patch)

3.87 ± 0.4

7

7.71 ± 2

9

19

0.2

2.92 ± 0.7

5

5.35 ± 1

6

4

0.05

activity at min. 15 of dopamine [%] (perforated patch)

43.11 ± 11.9

5

11.55 ± 7.6

9

39

0.02

12.13 ± 6.1

7

12.59 ± 5.4

12

49

1

 

in 300 nM isradipine

in 300 nM isradipine

 

juvenile WT

n

juvenile CaV1.3 KO

n

U

p

*adult WT

n

adult CaV1.3 KO

n

U

p

afterhyperpolarisation [mV] (whole-cell)

−56.94 ± 1.2

14

−56.44 ± 1.3

14

88

0.7

−59.81 ± 1.3

12

−59.3 ± 1.5

11

53

0.4

pacemaker frequency [Hz] (five minutes, perforated patch)

2.57 ± 0.04

7

1.96 ± 0.1

9

8

0.01

3.19 ± 0.3

9

    

CV ISI [%] (perforated patch)

8.85 ± 1.5

8

15.53 ± 3.5

8

17

0.1

9.9 ± 1.2

8

    

CV2 [%] (perforated patch)

3.73 ± 0.8

8

8.88 ± 2.6

8

12

0.04

5.7 ± 0.7

8

    

activity at min. 15 of dopamine [%] (perforated patch)

34.29 ± 9.9

7

5.39 ± 2.5

9

9

0.01

1.5 ± 3.2

13

    
 

in 10 μM Z941

in 10 μM Z941

 

juvenile WT

n

juvenile CaV1.3 KO

n

U

p

adult WT

n

adult CaV1.3 KO

n

U

p

afterhyperpolarisation [mV] (whole-cell)

−59.06 ± 0.8

17

−58.4 ± 1.6

14

118

1

      

pacemaker frequency [Hz] (five minutes, perforated patch)

2.19 ± 0.3

10

2.41 ± 0.3

8

31

0.5

2.4 ± 0.1

13

1.4 ± 0.1

10

6

<0.0001

CV ISI [%] (perforated patch)

12.44 ± 1.3

7

12.25 ± 1.4

7

20

0.6

7.05 ± 1.1

11

11.8 ± 1.2

9

12

0.003

CV2 [%] (perforated patch)

6.7 ± 1.1

8

6.8 ± 1.2

7

27

0.9

3.3 ± 0.4

10

7.1 ± 1.2

9

10

0.003

activity at min. 15 of dopamine [%] (perforated patch)

43.11 ± 14.3

8

42.6 ± 12.1

8

28

0.7

6.32 ± 3.2

13

13.66 ± 9.3

10

97

0.9

  

perforated patch/cell-attached in

 

10 mM EGTA (high EGTA, whole-cell)

10 μM DNIP

10 μM srDNIP

  
 

juvenile WT

n

juvenile CaV1.3 KO

n

U

p

juvenile CaV1.3 KO

n

juvenile CaV1.3 KO

n

U

p

afterhyperpolarisation [mV] (whole-cell)

−56.25 ± 0.7

15

−56.99 ± 0.8

19

116

0.4

      

pacemaker frequency [Hz] (five minutes)

1.97 ± 0.2

6

2.1 ± 0.2

7

18

0.7

2.6 ± 0.3

11

2.49 ± 0.4

10

50

0.7

CV ISI [%]

11.21 ± 1.1

6

11.31 ± 2

7

17

0.6

12.51 ± 1.7

8

9.12 ± 1.6

4

10

0.3

CV2 [%]

7.05 ± 1

6

6.27 ± 0.9

7

16

0.5

8.06 ± 1.7

8

7.13 ± 1.9

4

14

0.8

activity at min. 15 of dopamine [%]

65.2 ± 18.1

6

66.63 ± 13.5

7

26

0.6

28.95 ± 11.1

6

8.43 ± 8.4

7

7

0.03

  1. Presented are mean values ± SEM for afterhyperpolarisation (AHP), basal pacemaker frequency, pacemaker precision (given as coefficient of variation of the interspike interval, CV ISI, as well as CV2 values) and the relative activity at the last minute (min 15) in dopamine in respect to basal pacemaker frequency for SN DA neurons from juvenile and adult WT and Cav1.3 KO mice, under the distinct recording conditions, as indicated. min. = minute, U = U-value from Wilcoxon-Mann-Whitney-U two-sample rank-sum test (WMWU), p = p-value from WMW-test. Additional significant differences according to Kruskal-Wallis and post hoc Dunn’s Test for multiple comparisons (DTMC): AHP: juvenile WT vs. juvenile WT high EGTA: DTMC p = 0.04; juvenile Cav1.3 KO vs. adult Cav1.3 KO: DTMC p = 0.01; mean frequency: juvenile Cav1.3 KO vs. adult Cav1.3 KO: DTMC p = 0.01; activity at last minute of dopamine application: juvenile Cav1.3 KO vs. juvenile Cav1.3 KO high EGTA: DTMC p = 0.007; pacemaker frequency: adult Cav1.3 KO vs. adult Cav1.3 KO in Z941: DTMC p < 0.0001. Additional significant differences according to 1-way ANOVA and Fishers LSD (FSLD) test: CV2: juvenile WT vs. juvenile WT Z941: FLSD p = 0.01; juvenile WT Z941 vs. adult WT Z941: FLSD p = 0.001; pacemaker frequency: adult WT vs. adult WT Z941: FLSD p = 0.01. *perforated patch data adapted from11.
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