Figure 2
From: Glucanocellulosic ethanol: the undiscovered biofuel potential in energy crops and marine biomass
Engineered saccharification and ethanol production of (1, 3)-β-glucan-rich biomass.
(a) Amounts of glucose and the oligosaccharides laminaribiose and –triose remaining in the fermentation broth after 48 h of maize leaf biomass fermentation comparing yeast strains MaV (non-adapted), CEN (adapted) and CEN+LBT (engineered). (b) In vitro growth assays of yeast strains CEN and CEN+LBT on substrates as indicated. (c) Amounts of laminaribiose and –triose (left panel) as well as glucose and the combined amount of these three (1, 3)-β-glucan hydrolysis products (right panel) as indicators for changes in saccharification efficiency of maize and Miscanthus leaf biomass due to additional F. johnsoniae (1, 3)-β-glucanase application (−/+ BGL) after standard biomass pretreatment. (d) Ethanol production after 48 h of fermentation using yeast strains CEN and CEN+LBT and −/+ BGL. Values represent the mean of three independent biological experiments. Letters a, b, c: groups with significant difference, P < 0.05 based on Tukey’s test. Error bars represent ± SE.
