Figure 2
From: Methylene Blue Inhibits Caspases by Oxidation of the Catalytic Cysteine
Michaelis-Menten kinetic analyses of phenothiazines on RCasp6.
Initial enzyme velocity of Casp6 plotted against indicated concentrations of Ac-VEID-AFC substrate in presence of 0, 10 μM and 25 μM methylene blue (a), 0, 0.2 μM and 0.4 μM azure A (b), or 0, 1 μM and 5 μM azure B (c). The inset represents the Lineweaver-Burk plot for methylene blue (a), azure A (b) and azure B (c) with Ac-VEID-AFC substrate showing competitive mode of inhibition. Data represent the mean ± S.D. of three independent experiments.
