Table 2 Kinetic parameters of Ac-SDKP hydrolysis by different ACE enzymes.
From: Structural basis of Ac-SDKP hydrolysis by Angiotensin-I converting enzyme
Enzyme | kcat (s−1) | Km (μM) | kcat/Km (s−1.M−1) |
---|---|---|---|
N-dom | 14.2 ± 1.2 | 199.6 ± 25.5 | (0.72 ± 0.06) × 105 |
C-dom | 4.8 ± 0.6 | 138.2 ± 20.3 | (0.35 ± 0.01) × 105 |
sACE | 12.7 ± 0.4 | 239.5 ± 29.4 | (0.54 ± 0.06) × 105 |
N-sACE | 34.0 ± 3.9 | 493.8 ± 79.3 | (0.70 ± 0.04) × 105 |
C-sACE | 8.2 ± 0.5 | 436.3 ± 33.4 | (0.188 ± 0.002) × 105 |
CC-sACE | 19.4 ± 0.8 | 503.8 ± 31.6 | (0.39 ± 0.03) × 105 |