Figure 2
NALT promoted cell proliferation in vitro and in vivo.
(A): The CCK8 detection in cells treated with NALT shRNA, GSI, NICD and the corresponding control after cells treated for 24h. Absorbance at 450 nm was presented as the mean ± SEM. (B): The absorbance was detected in 0h, 12h, 24h and 48h. (C,D): The EDU stain also performed in cells treated as described above with a magnification of 200. The integral optical density value of cells treated with control plasmids was normalized to 100%. E: subcutaneous injection in NOD/SCID mice with Jurkat stably expressed with NALT shRNA, GSI, NICD or control (n = 5). The volume of each tumor was calculated as the length × width2 × 0.5 nearly every 3 days. F: Mice with established tumors were imaged every 7 d in different groups by IVIS Lumina II system; shown are images taken at day 25. Data presented as the mean ± SEM (*indicated P < 0.05, **indicated p < 0.01).
