Figure 1
Characterisation of DNA Mobility with RICS and iMSD.
(A) The RICS approach requires an image collected with a raster scan and works on the principle that a slower moving particle will be observed across fewer pixels compared to a faster particle resulting in a spatial correlation that decays earlier (B). (C) Mobility coefficients are obtained from the SCAF fit, as shown for the 240 bp fragment (top) and linear plasmid lipoplex (bottom) in solution exhibiting a diffusion of 12 μm2/s and 0.8 μm2/s, respectively. (D) Diffusion of naked DNA (blue, filled) and DNA lipoplexes (red, empty) in solution obtained from RICS analysis. In the graph presented, the diamonds represents the naked and lipoplex circular plasmid in blue and red, respectively. (n = 10 samples and 10 observations for each). (E) Through the same image series collected for RICS, the iMSD approach can be applied enabling the mechanism of motion to be determined through the calculation of the MSD. Mechanisms of motion include random diffusion, active transport, anomalous subdiffusion, confined diffusion and transient confinement (left to right).
