Figure 2 | Scientific Reports

Figure 2

From: Microarray Profiling and Co-Expression Network Analysis of LncRNAs and mRNAs in Neonatal Rats Following Hypoxic-ischemic Brain Damage

Figure 2

Gene expressions.

(A) Comparison between microarray and qRT-PCR results. Four differentially expressed lncRNAs were validated by qRT-PCR. The columns in the chart represent the absolute fold-change between the groups (HI/sham) detected by microarray and qRT-PCR. The bars represent standard errors. The qRT-PCR results were closely correlated with the microarray data. *P < 0.05. (B) Knockdown efficiency of BC088414 specific siRNAs in PC12 cells. siRNA-1447 (siR-1447) and combination of all 3 siRNAs (siR-M) significantly inhibited BC088414 expression. *P < 0.05 versus Blank group. (C) Inhibition of BC088414 reduced expression of Adrb2 and Casp6 in PC12 cells following OGD treatment. The expression of BC088414, Adrb2 and Casp6 were overexpressed in PC12 cells after OGD. Knockdown of BC088414 abated the increase of Adrb2 and Casp6 in OGD treated PC12 cells. *P < 0.05 versus NC group. #P < 0.05 versus NC OGD group. Blank: non-transfected group; NC: nonsense siRNA transfected group; OGD: oxygen and glucose deprivation; NC OGD: nonsense siRNA transfected PC12 cells following OGD; siRNA OGD: siR-M transfected PC12 cells following OGD.

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