Figure 5
Voltage-gated Ca2+ currents are enhanced by ODM treatment and even more markedly by PEMF exposure.
Electrophysiology. (A) Histogram showing the percentage of cells expressing Ca2+ current, in the four different populations tested. (B) Histogram representing the mean Ca2+ current amplitude. Values are plotted as mean ± SE. The significance of the differences obtained is reported as *(P < 0.05) (C) Voltage dependence of steady-state activation of Ca2+ current. Normalized conductances were plotted as a function of voltage and fitted to a Boltzmann equation (see equation [1]). In the representative cell shown in (C), best fit parameters were V1/2 = 18.67 mV and k = 6.26. (D) Pharmacological analysis of Ca2+ currents. Currents were elicited by applying the same protocol described in Fig. 4E and then challenged with BayK8644. Steady-state current amplitude values were reported in the figure as a function of the voltage for the control current and after the addition of BayK. (E) Ca2+ channel immunodetection. Fluorescence microscopy images showing localization of L-type VGCC (green) and Osteocalcin (red). Nuclear chromatin was stained with DAPI (blue). The bars are 100 μM in length.
