Figure 2
From: Impairment of T cell development and acute inflammatory response in HIV-1 Tat transgenic mice
Aberrant thymopoiesis in Tat-Tg mice.
(A) Thymus and spleen morphology of wild type and Tat-Tg mice. Scale bar is indicated. (B) Thymus and spleen weight of wild type and Tat-Tg mice. Values (mean ± SE, n = 6) are shown (C) Total thymocytes and splenocytes number of 8 weeks old wild type and Tat-Tg mice. Values (mean ± SE, n = 6) are shown. (D) Thymocytes (1 × 106) of 8 weeks old wild type and Tat-Tg mice were stained with CD4-PE and CD8a-FITC. Each plot represents 20,000 events of a representative experiment with the percentage of thymic sub-populations. (E) Absolute number of thymic sub-populations of wild type and Tat-Tg mice. Values (mean ± SE, n = 6) are shown. The asterisk indicates a statistically significant difference among wild type and Tat-Tg mice, according to the Student’s t-test (p < 0.01). (F) Thymocytes (3 × 106) of 8-weeks old wild type and Tat-Tg mice were stained with anti-CD3-FITC, CD4-FITC, CD8-FITC, CD25-APC and CD44-PE antibodies. Triple negative (CD3−CD4−CD8−) population was gated on 500,000 events by FACS and analysed for the expression of CD44 and CD25. Percentages of thymic sub-populations are indicated. (G) Absolute numbers of DN1, DN2, DN3 and DN4 thymic sub-populations of wild type and Tat-Tg mice were calculated. Values (mean ± SE, n = 6) are shown. The asterisk indicates a statistically significant difference among wild type and Tat-Tg mice, according to the Student’s t-test (p < 0.01).
