Figure 2

Optimization of lentivirus concentration/storage conditions.

(a) Summary graphs of transduction efficiency as a function of storage duration at 4 °C. The percentages (black) and mean intensities (red) of the transduced cells were plotted to reflect the effectiveness of the raw (left) or concentrated (right) lentivirus. (b) Summary graphs of percentages (black) and mean intensities (red) of the HEK293T cells infected with the virus subject to multiple freeze-thaw cycles. (c) Representative images (left) and summary graph (right) of the flow cytometric analysis of the HEK293T cells transduced with the GFP-expressing lentivirus purified from virus-containing medium collected from the first 48 hours or 48–72 hours after transfection. All summary graphs show mean ± SEM; n = 3 independent experiments (***p < 0.001).