Figure 3

The lentivirus purified with 10,000 g centrifugation mediates the GFP expression in primary hippocampal neurons.

(a) Representative brightfield (left), fluorescent (middle) and merged (right) images of cultured hippocampal neurons infected with the GFP-expressing lentivirus purified with 10,000 g centrifugation. Scale bar: 40 μm. (b) Representative traces (left), mean frequencies (middle) and amplitudes (right) of mEPSCs (top) and mIPSCs (bottom) recorded from neurons either infected with the concentrated GFP-expressing lentivirus (1:50 concentration, 4 μl) or treated with an equal amount of PBS at DIV 4. (c) Representative traces (left), mean frequencies (middle) and amplitudes (right) of mEPSCs (top) and mIPSCs (bottom) recorded from neurons infected at DIV 10. All summary graphs show mean ± SEM; n = 3 independent experiments.