Figure 4
From: An optimized method for high-titer lentivirus preparations without ultracentrifugation
The lentivirus purified with 10,000 g centrifugation mediates the GFP expression in mice hippocampus.
1 μl virus (0.97 × 108 TU/ml) was injected unilaterally into the hippocampus of new born mice pups (P0) and GFP expression was examined 12 days after injection in the cryostat sections. Low (upper panels in (a,b) Scale bar = 200 μm) and high (lower panels in (a,b) Scale bar = 50 μm) magnification views of brain sections show GFP-expressing neurons in CA1 and CA3 regions of hippocampus.
