Figure 3
From: Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system
Detection of sgRNA:Cas9-mediated targeting in different tissues.
(a) Dead goat #D24 was chose for tissue distribution analysis of on –target mutations. PCR products of the targeted regions of MSTN and FGF5 from seven tissues. (b) Detection of sgRNA:Cas9-mediated on-target cleavage of MSTN and FGF5 by T7E1 cleavage assay. All PCR products from (a) were subjected to T7E1 cleavage assay. All the samples were digested by T7E1, suggesting that all the 7 tested tissues carry MSTN and FGF5 mutations.
