Figure 2
Disruption of calcium homeostasis and alteration of mitochondrial potential for selected drugs and drug pairs.
(A) Time lapsed capture of calcium dependent Fura −2 fluorescence for two live side-by-side intraerythrocytic strain Dd2 parasites showing rapid loss of digestive vacuole (D.V.) Ca2+ (bright green inner circle, top panels) upon perfusion with cytocidal (2 × LD50) dose of CQ (see methods). (B) Examination of the combination responses of KN-62 and Artemether (ATM) in three parasite lines via an isobologram analysis of the mitochondrial membrane potential as judged by a combination JC1 assay (panel 1), heatmap analysis of the viability combination response (10 × 10 matrix) (panel 2), Delta Bliss analysis (panel 3) and isobolographic analysis of the viability combination response (panel 4).
