Figure 3
Analysis of autophagosomal body puncta formation and trafficking in response to environmental and/or pharmacological stress.
Imaging and quantification of PfAtg8 containing puncta within parasite (the Plasmodium falciparum HB3 strain) infected RBC after 6 hour bolus exposure to Artemether (ATM) or ATM combinations: (A) ATM at the defined IC50 value (23 nM). (B) ATM at the defined LD50 value (80 nM). (C) ATM and Lumefantrine (LUM) at their respectively defined LD50 values (80 nM and 323 nM, respectively). (D) ATM at the defined LD50 value and GSK-2126458 at the defined LD50 value (102 μM). (E) ATM at the defined LD50 value and NVP-BGT226 at the defined LD50 value (18 nM). Panel 1: transmittance image. Panel 2: anti-PfAtg8 peptide antibody imaging (ex: 450-490, em: 500 to 550). Panel 3: DAPI nuclear staining (ex: 340–380, em: 450 to 490). Panel 4: a merged image of all three views.
