Figure 2
From: Lyophilization protects [FeFe]-hydrogenases against O2-induced H-cluster degradation
O2-sensitivity of lyophilized and dissolved [FeFe]-hydrogenases.
Sample annotations are as in Fig. 1 and H2-activities were determined accordingly. Hydrogenase proteins were (a) exposed after lyophilization to 100% O2 for up to 24 hours (green symbols, HYDA1; brown symbols, CPI) or to ambient air for 24 h (triangles) at room temperature or (b) solutions of non-lyophilized proteins (6.33 nM) were incubated with the indicated O2-levels for 15 min at 37 °C. Values are the mean of at least 3 independent measurements, error bars give standard deviations.
