Figure 10

DGLHD induced PD-L1 expression and reduced the ability of DCs to stimulate T lymphocytes proliferation.

(A) The PD-L1 mRNA expression in BMDCs. BMDCs came from 12 week-old NOD mice treated with DGLHD for 4 weeks from 8 until 12 weeks of age or model control animals (n = 3). (B) DGLHD down-regulated allogeneic MLR in vivo. DCs generated from NOD mice treated with or without DGLHD (n = 3). (C) DGLHD or active ingredients down-regulated allogeneic MLR in vitro. DCs generated from NOD mice treated with DGLHD or active ingredients for 48 h (n = 3). Data were presented as means ± SD. ^*P < 0.05, ^**P  <0.01 compared with the model control/control group. ^##P < 0.01 compared with DGLHD group. ^ΔP < 0.05, ^ΔΔP < 0.01 compared with the T lymphocytes without DCs stimulation.