Figure 3
OMA1 genetically interacts with supercomplex-stabilizing factors.
(A) BN- and SDS-PAGE analysis of mitochondria from log-phase oma1Δ cells expressing OMA1, overexpressing RCF1 or COX5a. Source data (full-length blots) are available online in Supplementary information. (B) Mitochondria from log-phase wild-type (WT), oma1Δ rcf1Δ and rcf1Δ oma1Δ cells were analyzed by native (70 μg of mitochondria) or denaturing (20 μg of mitochondria) PAGE. (C) Indicated strains were cultured in YPD medium for 0.5 (12 hours) and 8 days at 28 °C, spotted onto YPD (Glucose) plates and incubated at 28 °C or 37 °C. Pictures were taken after 2 days of growth. (D) The indicated strains were dropped onto YPD (Glucose), YPGal (Galactose) and YPGL (Glycerol/Lactate) plates and incubated at 28 °C.
