Figure 1 | Scientific Reports

Figure 1

From: Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

Figure 1

T-DNA organization of the binary plasmid used, pJS-M14.

RB is right border; LB is left border; there are two recombination-sites (RS) and in between them there are 3 genes (combinations), i.e. Recombinase R-LBD, codA-nptII and gfp. Outside the RS sites there is the marker gene gusintron, acting as gene-of-interest. The gusintron and gfp were both driven by apple 1.6 kb Rubisco promoter and apple Rubisco terminator (Schaart et al., 2011). After recombination, the genes between the RS sites will be removed, while the gusintrongene will remain. The unique restriction site EcoRI is used for digestion prior to Southern blotting; thegfp gene is the target for probing.

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