Figure 2 | Scientific Reports

Figure 2

From: Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

Figure 2

Southern blot and qPCR analysis of the selected T0 lines.

To evaluate the t-DNA insertion number in T0 plants of Line 1 and Line 2, Southern blotting analysis was conducted on the pooled genomes DNA sample of T1 progeny plants of them respectively, as showed in (Chart A) with WT as control. The outer right lane shows a molecular weight marker. Hybridizing fragments should have a minimal size of 2.8 kb. The (Chart B) provides the qRT-PCR data on the expression of the nptII gene and the codA gene in the in vitro leaf material of the two selected original T0 plants without any treatment. The average relative expression level of the highest performing line (Line 2 for both genes) was set at 100%. Statistical analysis doing a T-test (student t-test) showed that the difference in expression between both lines was significant (p < 0.01) for both genes.

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