Figure 5
The efficiency of 5-FC selection.
The efficiency of 5-FC selection is demonstrated by plotting survival at different step, 3 to 7 against the theoretical multiplication rate that can be achieved without any selection. Blue and red lines (with Y axis on the left) display the dynamics of shoot survival for respectively line 1 and line 2. The start value was calculated as the total number of shoots starting 5-FC selection, from the axillary bud regeneration, divided by the number of initial rooting plants times three as the number of axillary buds isolated from them. The start number for line 1 is nine and for line 2 is six. As start value for the ideal curve eight was taken which is close to the average start-value of line1 and line 2. The curve in green (with Y axis on the right) shows the ideal shoot multiplication curve starting from eight shoot in the beginning, assuming that one shoot in regeneration medium for 3 weeks without selection will produce 3 shoot clusters on average. Then, after 4 rounds of shoot to shoot amplification, there will be 648 shoots finally.
