Figure 7
T1 seedlings tested for the absence of the T-DNA fragment in between the recombination sites.
T1 seedlings originating from one of the Line 2, 15 μM DEX-treated recombinant were used for the tests showed in this figure. WT is the wild type control; Non- recombinant (T1) shows the T1 seedlings from the line 2T0 plant without DEX and 5-FC treatment. The teste for the presence of the visual markers, GUS and GFP were showed in (Chart A). All of seedlings shown were etiolated because that they come from seeds germinated and grown in the dark. The PCR test for the absence of the T-DNA fragment in between the recombination sites was showed in (Chart B). For the PCR the forward primer was located in between the recombination sites the reverse was outside. The length of amplification product is 252 bp. The number showed the lanes, 1 to 5 were the recombinants, 6 was WT and 7 was Non- recombinant T1. The +/− (+: positive; –: negative) underneath were the results of GUS staining on the same plants.
