Figure 9
The process in which wild-type vegetation tissues were identified from a chimeric crambe GM shoot.
This figure exemplifies the process in which wild-type vegetation tissues were identified from a chimeric shoot of line 2 after the 15 μM DEX treatment and 5-FC selection. There were 3 axillary buds obtained from the shoot in total. As showed in the right corner, the blue arrows mean a round of regeneration and selection with 5-FC; plants in pale represent those killed by the selection and green plants indicate survivors. The drawn numbers are the actual number of plants handled. All the survivors were tested by Gus-staining at the end of Step 6, ‘O’ means GUS positive and ‘X’ means negative. So, finally from this specific starting plant, five surviving shoots obtained were GUS-negative and three were GUS-positive. And this result gives a thorough indication of the chimeric nature in the starting shoots.
