Table 4 GUS staining and PCR tests on T1 crambeseedling obtained from the recombinant shoots identified earlier.

From: Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

Seedlings

GUS staining

PCR

Blue

White

gfp

nptII

Negative

Positive

Negative

Positive

T1Family1

19

1

20

0

20

0

T1Family2

18

2

20

0

20

0

Line 2 (Without treatment)

18

2

1*

19

1*

19

  1. Note: Two T1 families (T1 Family 1 and T1 Family 2) from marker-free shoots that were confirmed twice by PCR to be marker-free (shown in Figure 7) were selected. T1 seedlings from the Line 2 shoot without DEX treatment and 5-FC selection were used as control. From each of the families, 20 seedlings were prepared for GUS staining and PCR testing respectively. The asterisks indicate that the results were on the same individual seedling.
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