Figure 4
Dynamics of SRV-4* infection in humanized mouse model (experiment 3).
(a-c) Virus isolation from infected humanized mice. (a) The human MNCs isolated from the BM and spleen of SRV-4-inoculated mice were cocultured with 293T cells. DNA was extracted from the cocultured 293T cells and proviral DNA was analyzed by PCR. (b,c) LacZ marker rescue assay. The detailed procedure is described in Methods and representative results of LacZ marker rescue assay are shown in c. Arrowheads indicate the presence of blue foci. (d,e) Proviral DNA (d) and viral RNA (e) were respectively analyzed by PCR and RT-PCR (targeted to env region). Actb/ACTB were used as internal control, and–RT was used as negative control. (f-n) Longitudinal analyses on the dynamics of SRV-4* infection. X-axes indicate wpi. (f) The body weights were routinely measured and were shown as the ratio to the initial weight. (g-n) The numbers of white blood cells (g), red blood cells (h), platelets (i), hematocrit (j), hemoglobin concentration (k) and the values of MCV (l), MCH (m) and MCHC (n) were measured by hematometry. In f-n, brown, SRV-4-infected mice (i.e., viral DNA was detected; no. 19 and 21); and black, SRV-inoculated mice without viral replication. The data from mock-infected mice (n = 3) were presented as the averages with SEMs. The mouse numbers correspond to those in Table 1. In a, d and e, gels have been cropped; full uncropped gels are available as Supplementary Figure 3.
