Figure 1

(a) Sketch of the 12 helix bundle DNA origami (220 × 14 × 14 nm3) equipped with a red ATTO647N dye and a fluorescent protein attached by hybridizing a DNA modified eYFP to a single stranded protrusion. (b) Corresponding TIRF image of overlaid channels of eYFP-emission (green) and ATTO647N-emission (red). Colocalized dyes appear yellow in the false-color image, scale bar 2 μm. (c) Representative single-molecule fluorescence transient colocalized eYFP and ATTO647N measured in dSTORM-buffer.