Figure 1
From: GPR120: A bi-potential mediator to modulate the osteogenic and adipogenic differentiation of BMMSCs
GPR120 positively regulates the osteogenesis of BMMSCs.
BMMSCs were cultured for 0, 3, 7, 14 and 21 days of osteogenesis, respectively. (A) RT-PCR and (B) western blot analysis of GPR120. BMMSCs were invalidated for GPR120 expression or transfected with a nontargeting vector. (C) Immunocytochemistry shows the expression of GPR120 (red) by BMMSCs. The merged image shows GPR120 with DAPI nuclear staining. (D) RT-PCR and (E) western blot analysis of GPR120. The expression of each target gene was calculated as the relative expression to β-actin and is represented as normalized fold expression. Data are represented as the mean ± SD of 3 independent experiments. *P < 0.05 and **P < 0.01 compared with the control group.
