Figure 2

Lack of GPR120 negatively impacts the osteogenesis of BMMSCs.

Control and GPR120 shRNA BMMSCs were incubated for 14 days of osteogenesis in the presence or absence of 50 μM TUG-891. (A) ALP (scale bar = 200 μm) and (B) Alizarin red (scale bar = 80 μm) staining of cells. (C) Difference in the ALP activity of osteogenic differentiation. The control value for ALP activity was 0.415 ± 0.020 units/mg protein. (D) Difference in the mineralization of osteogenic differentiation. The control value for mineralization was 0.910 ± 0.013 OD. (E) Difference in the osteogenic mRNA expression of Alp, Runx2 and Ocn. The expression of each target gene was calculated as the relative expression to β-actin and is represented as normalized fold expression. Data are represented as the mean ± SD of 3 independent experiments. *P < 0.05 and **P < 0.01 compared with the control group.