Figure 5

Low concentrations of TUG-891 induce the adipogenesis of BMMSCs.

BMMSCs were incubated for 3 days of adipogenesis in the presence of different concentrations of TUG-891 [0 (Con), 0.1, 0.5, 1 and 5 μM]. (A) Effect of low concentrations of TUG-891 on oil red staining (scale bar = 40 μm) and (B) quantification of lipid numbers of adipogenic differentiation in BMMSCs; the lipid quantification of each group was carried out using the method described in the Materials and Methods section. (C) Effect of low concentrations of TUG-891 on the adipogenic mRNA expression of Pparγ and Fabp4. (D) Western blot analysis of Erk1/2, pErk1/2, Ras, Ras-GRF, p38 and pp38. The expression of each target gene was calculated as the relative expression to β-actin and is represented as normalized fold expression. Data are represented as the mean ± SD of 3 independent experiments. *P < 0.05 and **P < 0.01 compared with the control group.