Figure 6

GPR120 activates different integrin family members to modulate osteogenesis or adipogenesis in a ligand dose-dependent manner.

BMMSCs were incubated for 14 days of osteogenesis or 3 days of adipogenesis in the presence of different concentrations of TUG-891 (0, 0.5, 50 μM). (A) Western-blot analysis of integrin α1, α2, αV, β1 and β3 of control BMMSCs. (B) Western-blot analysis of integrin α1, α2, αV, β1 and β3 of GPR120 knocked-down BMMSCs. (C) Molecular mode of GPR120 targeting the osteogenesis and adipogenesis of BMMSCs. Arrows indicate to activate the target. The repression symbol indicates the inhibition of the target. The green and blue arrows indicate the pathway of the high concentration and low concentration of TUG-891, respectively, targeting GPR120 and their downstream targets.