Figure 2
From: Role of Src in Vascular Hyperpermeability Induced by Advanced Glycation End Products
Effects of AGEs on Src phosphorylation.
HUVECs were either (a,c) incubated with 100 μg/mL of AGEs for 10, 30, 60, 90, or 120 min, or (d,f) stimulated with 12.5, 25, 50, 100, or 200 μg/mL AGEs for 60 min. Specific antibody was used to detect P-Src 419 (a,d) and P-Src 530 (c,f) by western blotting. The effect of BSA alone on Src 419 phosphorylation was also detected as BSA control by incubating the cells with (b) 100 μg/mL of BSA for 10, 30, 60, 90, 120 min, or with (e) 12.5, 25, 50, 100, 200 μg/mL BSA for 60 min. Those incubated with culture medium were used as blank control. The ratio of immunointensity between the phosphorylation of Src (p-Src 419, p-Src 530) and total Src were calculated. n = 3, *P < 0.05 versus control.
