Figure 1

The number of CD8⁺NKT-like cells increased after immunization with GFP-DCs.

(a) The flow chart shows that C57BL/6 mice were immunized by 2 × 10⁶ LPS-pulsed GFP-DCs four times. The splenocytes were then isolated and panNK cells were enriched using a panNK selection kit. (b) The gating strategy used to identify NK and NKT cell subsets is shown. (c) The proportion of TCRβ⁺CD49b⁺ cells in panNK cells and the absolute number of TCRβ⁺CD49b⁺ cells in naïve mice and GFP-DC-immunized mice were compared. (d) The proportion of CD8⁺NKT-like cells in TCRβ⁺CD49b⁺ cells and the absolute number of CD8⁺NKT-like cells in naïve mice and GFP-DC-immunized mice were compared. (e) We isolated 1 × 10⁶ CD49b⁺CD8 T cells and 1 × 10⁷ CD49b⁻CD8 T cells from B6 CD45.1 mice and B6 Thy1.1 mice, respectively, through flow cytometry; the cells were injected into C57BL/6 mice, which were subsequently immunized with 2 × 10⁶ GFP-DCs. After 14 days, the recipient mice were again immunized with 2 × 10⁶ GFP-DCs. Next, donor splenic cells were analyzed for phenotype and the CFSE dilution experiment was performed after 3 days. The data represent three independent experiments (n = 8–10).