Figure 2
From: Microgravity promotes osteoclast activity in medaka fish reared at the international space station
Mineralization status of the pharyngeal bone at day 56.
(A) Soft X-ray analysis. Lateral views of the heads of ground (left) and flight medaka (right) reared for 56 days. The red-dotted box in (Bag1-1 ground and Bag1-2 flight) shows the pharyngeal bone region depicted in “B.” Scale bar indicates 1 mm. (B) Magnified view of the red-dotted box in “A.” The mineralized area (white) was selected and outlined in yellow by using “ImageJ” software. Scale bar indicates 500 μm. The left-hand side of each figure is rostral. (C) Comparison of “mean gray value” as the mineralized signals at the pharyngeal region in the ground and flight medaka, normalized with the value of the background. *P < 0.05, by Student’s t-test; ground n = 6, flight n = 5. Error bars, s.e.m. (D) Mineral density of pharyngeal bone measured by pQCT. *P < 0.05, by Student’s t-test; ground n = 6, flight n = 5. Error bars, s.e.m. (E) Graded-color images for focal average thickness of the ventral inner part of the upper pharyngeal bone. Thicker places are shown in red; and thinner ones, in green. The color bar shows the thickness parameter in which red color indicates 0.085 mm and blue color, 0 mm. Scale bar represents 100 μm.
