Figure 1
From: Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters
Hypoionic shock potentiate aminoglycoside antibiotics to eradicate E. coli persisters.
(a) Plated stationary phase E. coli cells after being treated for 1 hour with tobramycin-containing (500 μg/ml) solutions of glycerol (2 M), NaCl (1 M) or distilled water. Data are representative of three independent experiments. (b,c) Survival ratio (in log scale) of the stationary phase E. coli cells after being treated for 1 hour with tobramycin-containing (500 μg/ml) solutions of the indicated osmolarity of NaCl or glycerol (b) or solution of mixed NaCl and glycerol (c). The data in (b,c) represent mean ± s.d. of three independent experiments. Asterisks indicate no detection of survival. The osmolarity of the LB medium is estimated to be around 0.4 Osm/L.
