Figure 3
From: Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters
The bacterial cell death likely occurs upon the reawakening of the persisters.
(a) Survival ratio of stationary phase MC4100 (streptomycin-resistant) or BW25113 (streptomycin-sensitive) E. coli cells after being treated with tobramycin- (500 μg/ml) or streptomycin-containing (2 mg/ml) distilled water. Asterisks indicate no detection of survival. (b) Time-dependent fluorescence-activated cell sorting analysis (left) and direct fluorescent microscopy imaging (right; at the 30 minutes point) of stationary phase cells that were treated with tobramycin-containing (500 μg/ml) distilled water before incubated with propidium iodide (50 μg/ml) in PBS or LB for the indicated length of time. A.U., arbitrary units. (c) Regular micrographs of the cells taken at the indicated time point. Scale bar, 5 μm.
