Figure 6
From: Jagged-2 enhances immunomodulatory activity in adipose derived mesenchymal stem cells
Immunologic characteristics of chondrogenic differentiated and/or IFN-γ pretreated AMSCs are dependent on up-regulation of Jagged-2.
Jagged-2 mRNAi transfection efficiency of chondrogenic differentiated and/or IFN-γ pretreated AMSCs was measured using several methods (A–D). (A) Fluorescent microscopy. (B) FACS. (C) Real-time PCR. (D) Western blot. (E) The proliferation of allogeneic lymphocytes stimulated in MLC experiments of chondrogenic differentiated and/or IFN-γ pretreated AMSCs was enhanced after Jagged-2 mRNAi transfection (*P < 0.05). (F) Chondrogenic differentiated and/or IFN-γ pretreated AMSC proliferation was inhibited following PHA stimulation of lymphocytes (*P < 0.05).
