Figure 2

Full wavelength scanning results.
(A) SDS-PAGE analysis of expressed GDO in BL21 (DE3) on a 12.5% gel. Lane M, protein molecular weight marker (MBI); Lane 1, uninduced cells; Lane 2, cell culture induced with 1 mM IPTG; Lane 3, the supernatant of the induced cells; Lane 4, the precipitate of the induced cells; Lane 5, purified recombinant His6-GDO protein; (B) NATIVE-PAGE of purified GDO-His6. Lane M, protein molecular weight marker (MBI); Lane 1, 5 μl purified GDO-His6.