Figure 6
Quantitative evaluation of the effects of the electrospun SF/PLCL nanofibrous scaffolds on RPC differentiation.
The cells were cultured under differentiation conditions. (A): Fluorescent micrograph of RPC cultures grown on SF:PLCL (1:1). Scale bars: 100 μm. (B): Scanning electron micrograph of RPC culture seeded on the SF:PLCL (1:1) nanofibrous scaffold. Scale bars: 20 μm. (C–E): The expression levels of rhodopsin (a marker of rod photoreceptor cells) and MAP2 (a marker of neuronal cells) were up-regulated 3.1-fold and 2.9-fold, respectively, whereas the expression of GFAP (a glial marker) was markedly down-regulated in the RPC cultures grown on the SF:PLCL (1:1) nanofibrous scaffolds compared with the levels observed in the control group. Notes: The error bars show the standard deviations (n = 3); *P < 0.05, **P < 0.01.
