Table 1 Primers used for quantitative qPCR.
Genes | Accession number | Forward (5′-3′) | Reverse (5′-3′) | Annealing Temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|---|
Ki-67 | X82786 | cagtactcggaatgcagcaa | cagtcttcaggggctctgtc | 60 | 170 |
nestin | NM_016701 | aactggcacctcaagatgt | tcaagggtattaggcaagggg | 60 | 235 |
Cadherin 4 | NM_009867.2 | atggttctgctgttcgttgtgt | ggtcgtagtcttggtcctcctc | 60 | 148 |
IL-6 | NM_031168.1 | aggagtggctaaggaccaaga | ataacgcactaggtttgccga | 60 | 100 |
MCP-1 | NM_011333.3 | acctgctgctactcattcacc | attccttcttggggtcagca | 60 | 148 |
Casepase-3 | NM_004346 | catggaagcgaatcaatggact | ctgtaccagaccgagatgtca | 60 | 139 |
Rhodopsin | NM_145383 | tcaccaccaccctctacaca | tgatccaggtgaagaccaca | 60 | 216 |
MAP2 | NM_001039934 | agaaaatggaagaaggaatgactg | acatggatcatctggtaccttttt | 60 | 112 |
β3-tubulin | NM_023279 | cgagacctactgcatcgaca | cattgagctgaccagggaat | 60 | 152 |
GFAP | NM_010277 | agaaaaccgcatcaccattc | tcacatcaccacgtccttgt | 60 | 184 |
β-actin | NM_007393 | agccatgtacgtagccatcc | ctctcagctgtggtggtgaa | 60 | 152 |
