Table 4 Partial results of partial least squares discriminant analysis, indicating for each source group the ten most important UNID-consensus spectra (ranked 1 to 10) that differentiated the isolates of the group from those of the other three groups.

From: A peptide identification-free, genome sequence-independent shotgun proteomics workflow for strain-level bacterial differentiation

Source group

Rank

No. of isolates

Replicate MS/MS spectra per isolate

Sequence tag

Sewage

Cow

Dog

Pig

Total

Sewage

Cow

Dog

Pig

Sewage

1

20

—

3

—

23

1.9

—

1.0

—

3

2

18

—

4

4

26

4.3

—

1.0

1.5

7

3

19

1

1

3

24

3.0

1.0

2.0

1.3

6

4

19

—

4

4

27

3.3

—

1.0

1.5

4

5

20

—

7

7

34

3.9

—

1.4

2.4

4

6

16

—

2

3

21

1.9

—

2.0

1.3

5

7

15

—

1

1

17

1.9

—

2.0

2.0

7

8

19

—

10

7

36

3.6

—

1.4

2.0

5

9

13

—

—

—

13

1.5

—

—

—

5

10

17

—

9

6

32

4.5

—

1.6

1.8

5

Cow

1

1

19

—

2

22

1.0

4.5

—

2.0

5

2

1

20

2

2

25

1.0

1.7

1.0

1.0

4

3

1

18

—

1

20

1.0

1.8

—

1.0

6

4

1

18

1

1

21

1.0

1.9

1.0

2.0

5

5

2

19

—

5

26

1.5

2.1

—

1.2

11

6

—

20

—

6

26

—

2.0

—

1.3

9

7

3

20

2

8

33

1.0

1.7

1.0

1.0

6

8

1

16

—

—

17

2.0

2.0

—

—

13

9

—

17

—

3

20

—

1.9

—

1.0

6

10

1

16

—

1

18

2.0

2.0

—

1.0

11

Dog

1

—

—

17

—

17

—

—

2.0

—

10

2

—

—

17

—

17

—

—

5.9

—

5

3

—

—

17

—

17

—

—

5.5

—

6

4

—

—

17

—

17

—

—

1.7

—

7

5

—

—

16

—

16

—

—

1.9

—

6

6

—

—

16

—

16

—

—

19.8

—

5

7

—

—

17

—

17

—

—

3.5

—

7

8

—

—

16

—

16

—

—

1.8

—

5

9

—

—

17

—

17

—

—

1.7

—

6

10

—

—

16

—

16

—

—

2.3

—

11

Pig

1

1

5

6

13

25

1.0

1.2

2.0

3.4

7

2

—

—

—

10

10

—

—

—

1.5

4

3

—

3

4

13

20

—

1.7

2.5

3.1

7

4

1

7

8

13

29

1.0

3.6

3.1

6.5

5

5

—

1

2

12

15

—

3.0

1.5

2.3

4

6

—

1

4

11

16

—

1.0

1.5

2.7

4

7

—

2

4

12

18

—

5.5

4.3

6.1

4

8

—

—

1

9

10

—

—

1.0

2.9

5

9

—

—

1

10

11

—

—

2.0

1.7

3

10

—

7

5

12

24

—

3.3

3.0

5.6

4

  1. Data shown for each UNID-consensus spectrum are (i) the number of isolates that it was associated with in each source group, (ii) the average number of replicate MS/MS spectra derived from each associated isolate in a group, and (iii) the length of the longest sequence tag (i.e. no. of amino acids) identified using de novo sequencing.