Figure 3
SETD7 regulates mitochondria stability through PPARGC1A.
(a) Mitochondria population was visualized by MitoTracker (green) in NHLFs with or without CSE stimulation. Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (b) Cell viability of NHLFs over time course with or without CSE-induced oxidative stress was assessed by WST-1. The tetrazolium salt WST-1 can be cleaved by mitochondrial dehydrogenases in live cells and form formazan dye in proportion to the number of viable cells. (c,d) DNA Ligase III (LIG3) mRNA level was quantified by qRT-PCR in Beas-2B cells with either ectopic expression of SETD7 or siSETD7 knockdown. CSE or H2O2 was added into the media to induce oxidative stress. (e) PPARGC1A mRNA in SETD7 knockdown Beas-2B cells treated by either CSE or H2O2 was measured by qPCR. (f,g) Western blot was performed to quantify PPARGC1A and SOD2 protein expression in the SETD7 knockdown Beas-2B cells under CSE-induced oxidative stress. Relative expression of both proteins was compared with Histone H3 control in each individual experiment. Data were expressed as mean ± standard error of the mean (SEM). n = 3. * indicates P < 0.05; ** indicates P < 0.01 (two-way ANOVA).
