Figure 6
SETD7 inhibitor is able to alleviate ROS accumulation.
(a) Quantification of CSE-induced mitochondrial ROS production with either DMSO or different doses of (R)-PFI-2 in NHLFs by MitoSox. (b) NHLFs viability under CSE treatment at the presence of (R)-PFI-2 was determined by WST-1 assay at 0- and 8-hour time point. ** indicates P < 0.01, *** indicates P < 0.001 (Student’s unpaired t test). (c–i) Transcription levels of NFE2L2, PPARGC1A, HMOX1, TXN, SOD2, CAT and LIG3 were assessed by qRT-PCR using total RNAs from Beas-2B cells treated with SETD7 inhibitor under CSE-induced oxidative stress. Data were expressed as mean ± standard error of the mean (SEM). n = 3. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (two-way ANOVA).
