SDS-PAGE in Fig. 5.

From: The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress

Spots	Protein name	Source	Accession No	Theoretical Mr/PI	Score
1	ATP synthase CF1 alpha subunit	Atropa belladonna	gi\|28261702	55476.1/5.26	355
2	ATP synthase beta subunit	Cucumis sativus	gi\|14718022	52002.1/5.13	655
3	ATP synthase beta subunit	Cucumis sativus	gi\|14718022	520021/5.13	640
4	putative magnesium subunit chlD	Oryza sativa Japonica Group	gi\|32129323	81025.0/5.38	47
5	LHCII type I chlorophyll a/b-binding protein	Vigna radiate	gi\|9587207	27950.1/5.13	114
6	LHCII type I chlorophyll a/b-binding protein	Vigna radiate	gi\|9587205	28003.1/5.14	149
7	chlorophyll a/b-binding protein CP26 in PS II	Brassica juncea	gi\|1644289	30572.9/6.00	78
8	putative elicitor-inducible cytochrome P450	Oryza sativa Japonica	gi\|51091420	59812.2/8.80	52
9	ATP synthase CF1 alpha subunit	Atropa belladonna	gi\|28261702	55476.1/5.26	409
10	ATP synthase beta subunit	Cucumis sativus	gi\|14718022	52002.1/5.13	739
11	F-ATPase subunit alpha	Nicotiana tabacum	gi\|55977763	55477.1/5.14	298
12	H(+)-transporting ATP synthase	Dais cotinifolia	gi\|4995109	52200.1/5.15	310
13	ATPase subunit I	Spinacia oleracea	gi\|7636090	47260.7/5.47	210
14	ATP synthase beta subunit	Cucurbita pepo	gi\|14718024	52043.0/5.04	167
15	photosystem II CP47 protein	Gunnera chilensis	gi\|27446459	53482.0/6.07	294
16	photosystem II CP47 protein	Roystonea princep	gi\|37721438	54951.7/6.02	188
17	photosystem II P680 chlorophyll A apoprotein	Oenothera argillicola	gi\|16914273	56076.5/6.40	347
18	photosystem II Qb protein	Bruguiera gymnorhiza	gi\|8131597	14347.0/4.71	170
19	psbA	Conocephalum conicum	gi\|5689006	38866.4/5.30	79
20	photosystem II CP43 protein	Chloranthus japonicas	gi\|27435869	47413.3/6.64	331
21	photosystem II CP47 protein	Dasypogon hookeri	gi\|37721411	55007.8/6.13	480
22	photosystem II protein D1	Lotus japonicus	gi\|13518418	39064.5/5.11	167
23	chlorophyll a/b-binding protein precursor	Citrus limon	gi\|28630973	22937.5/5.25	98
24	photosystem II CP47 protein	Gunnera chilensis	gi\|27446459	53482.0/6.07	244
25	ATP synthase beta subunit	Austrobaileya scandens	gi\|7687960	53571.8/5.15	48
26	chlorophyll a/b-binding protein CP24 precursor	Vigna radiata	gi\|8954298	27344.1/6.15	186
27	LHCII type III CAB-13	Solanum lycopersicum	gi\|115794	28661.4/5.09	80
28	Methyltransferase	Arabidopsis thaliana	gi\|15232158	31799.4/8.51	63
29	ATP synthase epsilon subunit	Nasa triphylla	gi\|22796597	14527.8/5.43	148
30	photosystem II D2 protein	Columnea sp. Lindqvist and Albert	gi\|22858666	14299.2/5.07	290
31	ATPase subunit I	Spinacia oleracea	gi\|7636090	47260.7/5.26	301
32	photosystem I P700 chlorophyll a apoprotein A2	Nicotiana tabacum	gi\|11465954	82470.8/6.72	89

Note: A MASCOT search against the NCBInr database revealed the identity of 29 of the 32 proteins. The remaining three proteins (spots 4, 8 and 25, Fig. 5) had matching scores significantly lower than the threshold and may represent unknown proteins. Theoretical molecular weight (Mr) and pI values were calculated using the ProtParam tool available at http://us.expasy.org/.

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Table 2 Differentially synthesised proteins identified by MALDI-TOF-MS and LC–ESI-MS/MS from the spots in BN/SDS-PAGE in Fig. 5.

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