Table 3 Primers used for qRT-PCR analysis and their target genes.
Gene | Product length (bp) | Annealing temp. (°C) | Primer sequence |
|---|---|---|---|
D1 | 167 | 60.1 | S 5′-TCTGCAGCTATCGGTTTGC-3′ |
59.7 | As 5′-GGACGCATACCCAGACAGA-3′ | ||
atpB | 160 | 60.0 | S 5′-GCGGGCCTATTCCATCTT-3′ |
60.2 | As 5′-TCGGGGTAGTTGCTGGAA-3′ | ||
atpA | 179 | 60.0 | S 5′-TGATGCCTGTCGCTGTGT-3′ |
60.0 | As 5′-AGTCGCCTCAAACGCAAC-3′ | ||
D2 | 186 | 60.0 | S 5′-TACCCTTCCTGCCATCCA-3′ |
60.1 | As 5′-TGCTCAAACGCTTGGTGA-3′ | ||
LHCII | 210 | 55.8 | S 5′-AGCCACCATCCTACCTTACC-3′ |
56.3 | As 5′-CAGCCTTGAACCAAACAGC-3′ | ||
CP43 | 182 | 58.6 | S 5′-GAATACGGTAAAGTGGCTCCTG-3′ |
50.0 | As 5′-CCTAAAGCACCTAAACTATAAGAC-3′ | ||
CP47 | 298 | 49.5 | S 5′-GGTCCTGGAATATGGGTA-3′ |
50.0 | As 5′-TTGCTGGAACTATGTGGTA-3′ | ||
Qb | 148 | 48.8 | S 5′-AAACTCTAATACAGGCATACG-3′ |
38.1 | As 5′-AAATCGGACACTTTTCTCA-3′ | ||
CP24 | 252 | 52.8 | S 5′-CTGGTGTTAGAGGTGGTGG-3′ |
57.9 | As 5′-CGAAGGAGAATGGAGCAA-3′ | ||
psbA | 249 | 48.5 | S 5′-CGGTGGTCCTTATGAGAT-3′ |
50.0 | As 5′-TTACGGTCGTTCCTTATTC-3′ | ||
actin | 290 | 58.0 | S 5′-CCGTTCTGTCCCTCTACGCTAGTG-3′ |
As 5′-GGAACTGCTCTTTGCAGTCTCGAG-3′ |
