Figure 4 | Scientific Reports

Figure 4

From: Enhanced nematicidal potential of the chitinase pachi from Pseudomonas aeruginosa in association with Cry21Aa

Figure 4

Recombinant plasmid construction and SDS-PAGE analysis of protein expression.

(A) Fusion protein construction and partial restriction enzymatic site. (B) SDS-PAGE analysis of recombination protein expression in E. coli and purification. The arrows point to target proteins. Lane 1: The standard protein molecular mass marker (14.4–116.0 kDa). Lane 2–5: Induced cell lysate supernatants of pGEX-6p-1, pGEX-6p-CHACry, pGEX-6p-Cry21Aa and pGEX-6p-pachi harbored in E. coli. Lane 6-8: Induced cell lysate sediments of pGEX-6p-CHACry, pGEX-6p-Cry21Aa and pGEX-6p-pachi harbored in E. coli. Lane 9–11: Purified CHACry, Cry21Aa and pachi proteins.

Back to article page