Table 1 Synergism and elevation in activity of pachi and Cry21Aa.

From: Enhanced nematicidal potential of the chitinase pachi from Pseudomonas aeruginosa in association with Cry21Aa

Nematicidal activity

Toxin

Regression coefficient

LC 50 (μg/ml) a

Synergistic factor (SF)

Actual value

Expected value

pachi

0.9651

387.3 (355.6–419.1)

  

Cry21Aa

0.9708

78.7 (73.2–84.2)

  

Combination protein(ratio)

 Cry21Aa:pachi(2:1)

0.9660

45.7 (41.9–49.5)

108.7

2.4

 Cry21Aa:pachi(1:1)

0.9663

44.0 (40.2–47.9)

154.3

3.5

 Cry21Aa:pachi(1:2)

0.9591

43.5 (39.2–47.8)

168.0

3.9

 Cry21Aa:pachi(1:3)

0.9544

30.9 (26.8–35.1)

195.1

6.3

 Cry21Aa:pachi(1:5)

0.9333

39.9 (34.5–45.4)

234.4

5.9

 Cry21Aa:pachi(1:8)

0.9617

53.3 (49.0–57.7)

269.5

5.1

Fusion protein CHACry

0.9805

37.8 (35.6–40.0)

154.3

4.1

  1. aThe real values were calculated as described by Bischof et al.52 with statistical significance set at p < 0.05. Expected values were calculated using the equation of Tabashnik et al.53.
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