Figure 2 | Scientific Reports

Figure 2

From: A whole-cell tumor vaccine modified to express fibroblast activation protein induces antitumor immunity against both tumor cells and cancer-associated fibroblasts

Figure 2

Induction of protective anti-tumor immunity.

C57BL/6J and BALB/c mice were immunized with pFAP-transfected, pVector-transfected, or non-transfected tumor cells or n.s. on day −28, −14, −7 and then challenged with 1 × 106 B16F10 cells, LL/2 cells or CT26 cells on day 0. (a,b) There was a significant difference in the tumor volume between the mice immunized with pFAP-transfected tumor cells and the control cells in the B16F10 melanoma (B16F10) and Lewis lung carcinoma (LL/2) models. (*P < 0.05 or **P < 0.01; ANOVA). (d,e) A significant increase in tumor-free survival of the group immunized with pFAP-transfected tumor cells, compared with other groups, was observed in the B16F10 and LL/2 models (P < 0.05). (c,f) The immunized mice were nearly tumor-free after challenge with CT26 cells, in contrast to the n.s.-treated mice. Points, mean (n = 10); bars, SD.

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